Simultaneous recording of hippocampal oxygen and glucose in real time using constant potential amperometry in the freely-moving rat

摘要: Abstract Amperometric sensors for oxygen and glucose allow real time recording from the brain in freely-moving animals. These have been used to detect activity- drug-induced changes metabolism a number of regions but little attention has given over hippocampus despite its importance cognition disease. Sensors were co-implanted into allowed record several days. Baseline recordings show that basal concentrations hippocampal are 100.26 ± 5.76 μM 0.60 ± 0.06 mM respectively. Furthermore, stress-induced neural activity shown significantly alter both analytes hippocampus. Administration O 2 gas animals’ snouts results significant increases administration N decrease oxygen. Chloral hydrate-induced anaesthesia causes increase whereas treatment with carbonic anhydrase inhibitor acetazolamide glucose. findings provide electrochemical data which previously impossible traditional methods such as microdialysis or ex vivo analysis. As such, these window function can be conjunction behavioural pharmacological interventions further elucidate functions mechanisms action normal disease states.