Detection of the FCGR3a polymorphism using a real-time polymerase chain reaction assay

摘要: The fragment crystallizable (Fc) region of the immunoglobulin G, low affinity III A receptor (FCGR3a, also known as CD16) belongs to Fc gamma family (FCGR), which plays an important role in immunoinflammatory processes. It is a affinity, transmembrane that mainly expressed monocytes, natural killer cells, and macrophages. has been implicated various inflammatory conditions, recently polymorphism (rs396991) this gene shown influence response rituximab (anti-CD20) therapy disorders. We evaluated two molecular methods genotype polymorphism. Archived, formalin-fixed, paraffin-embedded samples from 26 biopsies diffuse large B-cell lymphoma were retrieved DNA was extracted. tested for FCGR3a using real-time polymerase chain reaction (PCR) followed by melt curve analysis or standard TaqMan allelic discrimination assay ABI 7500 FAST PCR instrument. With assay, we found 16 cases wild type genotype, homozygous phenylalanine (F/F), receptor, whereas had valine (V/V) eight heterozygous with V/F genotype. Results similar 25 cases; however, four did not have sufficient method, result one sample discordant. new offers several advantages over previously published assays, such faster turnaround time ease interpretation. These performance characteristics make it highly suitable use clinical laboratory.