An electrochemical biosensor for highly sensitive detection of microRNA-377 based on strand displacement amplification coupled with three-way junction

摘要: Abstract In this research, a novel electrochemical biosensing strategy has been developed for detection of microRNA (miRNA) by integrating strand displacement amplification (SDA) with three-way junction (TWJ). The target miRNA triggers the SDA cycle polymerization, nicking and reaction in presence template hairpin structure containing recognition sites restriction enzyme its downstream linker sequence. This is amplified polymerization cleavage reactions, producing large quantities linkers. linkers are employed hybridization between TWJs capture probes, which can transform single signal readout into multiple output. Thus, obtained using streptavidin linked to alkaline phosphatase (ST-AP) toward synthetic substrate α-naphthyl phosphate (α-NP). proposed exhibits high sensitivity specificity dynamic range 1 fM 1 nM low limit down 0.68 fM also achieves acceptable reproducibility determination miRNA-377. presents promising platform highly sensitive miRNAs biomedicine early clinical diagnostic application.