Porous protein-based scaffolds prepared through freezing as potential scaffolds for tissue engineering.
作者： Linda Elowsson , Harald Kirsebom , Virginie Carmignac , Madeleine Durbeej , Bo Mattiasson
DOI: 10.1007/S10856-012-4713-4
关键词: Porous scaffold 、 Biophysics 、 Skeletal tissue 、 Biomedical engineering 、 Porosity 、 Tissue engineering 、 Regeneration (biology) 、 Casein 、 Materials science 、 Gelatin 、 Ovalbumin
摘要: Successful tissue engineering with the aid of a polymer scaffold offers possibility to produce larger construct and mould shape after defect. We investigated use cryogelation form protein-based scaffolds through different types formation mechanisms; enzymatic crosslinking, chemical non-covalent interactions. Casein was found best suited for gelatin ovalbumin Fibroblasts myoblasts were used evaluate cryogels purposes. The stability over time in culture differed depending on mechanism. showed potential be skeletal engineering, whereas would more suitable compliable soft tissues even though it also seemed support myogenic phenotype. Ovalbumin better elastic faster regeneration properties due its degradation time. Overall, technique fast, cheap reproducible way creating porous from proteins without toxic compounds.
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