Confocal microscopy of fertilization-induced calcium dynamics in sea urchin eggs
作者： Stephen A. Stricker , Victoria E. Centonze , Stephen W. Paddock , Gerald Schatten
DOI: 10.1016/0012-1606(92)90292-O
关键词: Biology 、 Fluorescence 、 Sea urchin 、 Confocal microscopy 、 Zygote 、 Nucleus 、 Human fertilization 、 Biophysics 、 Calcium 、 Cleavage (embryo) 、 Anatomy
摘要: Although confocal microscopy has typically been utilized in studies of fixed specimens, its potential for exploring dynamic processes living cells is rapidly being realized. In this report, laser scanning used to analyze the calcium wave that occurs following fertilization sea urchin eggs microinjected with calcium-sensitive fluorescent probes fluo-3 or green. Time-lapse recordings optical sections depicting dynamics within are also subjected volumetric reconstructions. Such analyses indicate (1) cytoplasmic free levels become elevated throughout fertilized egg, (2) causes egg nucleus undergo a transient increase calcium, and (3) normal cleavage can be obtained time-lapse imaging waves.
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