Detecting coevolution of positively selected in turtles sperm-egg fusion proteins.

摘要: Abstract Physically interacting sperm-egg proteins have been identified using gene-modified animals in some mammal species. Three are essential for binding: Izumo1 on the sperm surface, and JUNO CD9 egg surface. Most linked to reproductive function evolve rapidly among species by positive selection, correlated evolutionary rates compensate changes both egg. Up now, interactions between not non-mammalian vertebrates, such as turtles that interspecific hybrids can produce surviving F1 generations. To explore potential physical of turtle species, coding region Izumo1, JUNO, homologous genes (named Tu-Izumo1, Tu-JUNO, Tu-CD9) six (Mauremys reevesii, M. mutica, sinensis, Cistoclemmys flavomarginata, Platysternon megacephalum Chrysemys picta bellii) were identified, amplified, sequenced, tissue-specific expression was analyzed reevesii. We constructed phylogenetic trees signatures coevolution protein pairs MirrorTree Server linear regression methods. The results showed Tu-CD9 rates, area where Tu-Izumo1 interacts with Tu-JUNO has only one selection site These suggest there is a interaction interbreed, significantly lower may be reasons why so common. Further studies required uncover biological functions during gamete fusion.