High-affinity SV40 T-antigen binding sites in the human genome.

摘要: Abstract We describe two different approaches to isolate human genomic sequences possessing high-affinity binding sites for the simian virus 40 (SV40) large T antigen. First, SV40 antigen was added toSau3A-restricted DNA; resulting T-antigen-DNA complexes were collected after repeated passages through nitrocellulose filters. The second approach involves specific immunoprecipitation of chromatin fragments, generated bySau3A treatment nuclear from SV40-transformed cells. DNA fragments obtained cloned in plasmid vectors further investigation. Using filter we isolated four with sites. site one fragment related strong T-antigen 1 genome. other three contained multiple recognition pentamers, GA(G)GGC. Only a identified among immunoprecipitable fragments. This belongs L1 family repetitive DNA. present evidence suggesting that significant fraction elements possesses L1-related appear as extrachromosomal an cell line, and amount found increase fusion transformed cells permissive monkey