Genetic and epigenetic changes of NKIRAS1 gene in human renal cell carcinomas.
作者： Zabarovsky Er , Kashuba Vi , Rynditch Av , Zgonnyk Ym , Gerashchenko Gv
DOI:
关键词: Biology 、 Cancer research 、 Cancer 、 Renal cell carcinoma 、 Gene 、 Epigenetics 、 Microarray analysis techniques 、 Methylation 、 Copy-number variation 、 Sarcoma 、 Molecular biology
摘要: Renal cell carcinoma (RCC) is the most common malignant tumor of kidney associated with worst clinical outcome. No mo- lecular markers for RCC diagnostics and prognosis that could be applied in clinics were described yet. Large-scale screening 3p human chromosome genes/loci histologically normal tissues surrounding tumors using NotI-microarray approach demonstrated NKIRAS1 gene contained largest percent genetic/epigenetic changes cells. Aim: To validate results NotI microarray analysis study genetic, epigenetic changes, expression level samples. Methods: DNA RNA isolated from freshly-frozen renal tumors' samples (n = 12) tumors. Epigenetic (methylation status) detected by bisulfite sequenc- ing. Genetic analyzed Quantitative real-time PCR (qPCR) SYBR Green. For relative quantification 2-ΔΔCP method was used. Nonparametric tests (Wilcoxon, Kruskal — Wallis Mann Whitney) statistical data BioStat software. Results: downregulated 75% (9 compared tissue. High grade (3 4) showed lower at mRNA than low (1 2). significant association found between gender or age. Analysis copy number performed 19 Changes observed 64% 14) cRCC 9 displayed ratio ( 0.85) considered as number. all 3 benign oncocytomas, 1 papillary cancer sarcoma, where hemizygous deletion observed. methylation status RCC. Conclusions: We have validated It shown decreased this type tumor.
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