Nucleoside Transport Inhibition In Vitro and In Vivo
作者： ARP Paterson , ES Jakobs , CYC Ng , RD Odegard , AA Adjei
DOI: 10.1007/978-3-642-45619-0_8
关键词: In vivo 、 Transporter 、 Molecular biology 、 Nucleoside 、 Binding site 、 Efflux 、 Adenosine transport 、 In vitro 、 Chemistry 、 Cell culture
摘要: Among cultured cell lines, considerable diversity has been shown in the sensitivity of cellular nucleoside transport (NT) systems to nitrobenzylthioinosine (NBMPR), a potent inhibitor facilitated diffusion NT systems. In S49 lymphoma cells and human erythrocytes, are high NBMPR (IC50 1 μM). A number including leukemia L1210, appear possess both low NBMPR. We report joint presence hepatoma (Novikoff UASJ-2.9, Morris 3924A, Reuber H-35) with binding sites, finding which suggests that latter do not interact former. Implications these findings discussed. Recognition energy-requiring, concentrative several types added complexity this area. describe studies formycin B permeation into IEC-6 intestinal epithelial cells, show (a) system requires an inward Na+ gradient, (b) NBMPR-sensitive transporter mediates efflux.
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