Citral, a Monoterpene Inhibits Adipogenesis Through Modulation of Adipogenic Transcription Factors in 3T3-L1 Cells.

摘要: Obesity is considered as a major global human health problem which significantly increases the risk for development of type 2 diabetes. Citral, bioactive compound widely found in variety foods that are consumed daily. In this study, we investigated inhibitory effect citral against adipogenic genes 3T3-L1 cells. The mouse fibroblast pre-adipocytes were differentiated into adipocytes using cocktail (5 g/ml insulin, 0.5 mM isobutylmethylxanthine and 10 M dexamethasone). Differentiation was evaluated by assessing triglyceride accumulation assay cell viability MTT assay. PI3K/AKT signaling, specific transcription factors (PPARγ, SREBP-1c, FAS CPD) inflammatory (TNF-α, IL-6 MCP-1) analyzed western blotting reverse transcriptase PCR lines. increased induced cells, whereas treatment decreased levels concentration dependent manner. Further, shows there no reduction during differentiation expression PI3K/AKT, biomarkers MCP-1). Conversely, cells treated with suppress PPARγ, FAS, CPD, TNF-α, MCP-1 dose Thus, exhibits beneficial effects to inhibit adipogenesis through modulation markers.