Hypoxia increases human keratinocyte motility on connective tissue.
作者： E A O'Toole , M P Marinkovich , C L Peavey , M R Amieva , H Furthmayr
DOI: 10.1172/JCI119837
关键词: Laminin 、 Cell biology 、 Moesin 、 Connective tissue 、 Motility 、 Fibronectin 、 Integrin 、 Radixin 、 Biology 、 Keratinocyte
摘要: Re-epithelialization of skin wounds depends upon the migration keratinocytes from cut margins wound and is enhanced when human are covered with occlusive dressings that induce hypoxia. In this study, two independent assays were used to compare cellular motility on connective tissue components under normoxic or hypoxic conditions. Human apposed collagens fibronectin exhibited increased subjected (0.2 2% oxygen) conditions compared (9 20% When cells, expression redistribution lamellipodia-associated proteins (ezrin, radixin, moesin). Furthermore, demonstrated decreased secretion laminin-5, a laminin isoform known inhibit keratinocyte motility. Hypoxia did not alter number integrin receptors cell surface, but 92-kD type IV collagenase. These data demonstrate hypoxia promotes tissue. Hypoxia-driven associated lamellipodia proteins, collagenase locomotion brake for keratinocytes.
dm5migu4zj3pb.cloudfront.net 参考文章(62)
P Talke, A Fronek, E F Bernstein, F L Golbranson, U K Franzeck, Transcutaneous PO2 measurements in health and peripheral arterial occlusive disease. Surgery. ,vol. 91, pp. 156- 163 ,(1982)
Conlon Kc, Brennan Mf, DiResta Gr, Sclafani L, Comparison of transcutaneous oximetry and laser Doppler flowmetry as noninvasive predictors of wound healing after excision of extremity soft-tissue sarcomas. Surgery. ,vol. 115, pp. 335- 340 ,(1994)
J P Kim, K Zhang, J D Chen, R H Kramer, D T Woodley, Vitronectin-driven human keratinocyte locomotion is mediated by the alpha v beta 5 integrin receptor. Journal of Biological Chemistry. ,vol. 269, pp. 26926- 26932 ,(1994) , 10.1016/S0021-9258(18)47108-6
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Ronald N. Roth, Larry D. Weiss, Hyperbaric oxygen and wound healing Clinics in Dermatology. ,vol. 12, pp. 141- 156 ,(1994) , 10.1016/0738-081X(94)90265-8
K Pestonjamasp, M R Amieva, C P Strassel, W M Nauseef, H Furthmayr, E J Luna, Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes. Molecular Biology of the Cell. ,vol. 6, pp. 247- 259 ,(1995) , 10.1091/MBC.6.3.247
Christer Hammarlund, Thomas Sundberg, Hyperbaric oxygen reduced size of chronic leg ulcers: a randomized double-blind study. Plastic and Reconstructive Surgery. ,vol. 93, pp. 829- 833 ,(1994) , 10.1097/00006534-199404000-00026
Thomas K Hunt, The physiology of wound healing. Annals of Emergency Medicine. ,vol. 17, pp. 1265- 1273 ,(1988) , 10.1016/S0196-0644(88)80351-2
Janice P Kim, Ken Zhang, Randall H Kramer, Thomas J Schall, David T Woodley, Integrin Receptors and RGD Sequences in Human Keratinocyte Migration: Unique Anti-Migratory: Unique Anti-Migratory Function of α3β1 Epiligrin Receptor Journal of Investigative Dermatology. ,vol. 98, pp. 764- 770 ,(1992) , 10.1111/1523-1747.EP12499947
Guenter Albrecht-Buehler, The phagokinetic tracks of 3T3 cells Cell. ,vol. 11, pp. 395- 404 ,(1977) , 10.1016/0092-8674(77)90057-5