Elimination of mouse tumor cells from neonate spermatogonial cells utilizing cisplatin-entrapped folic acid-conjugated poly(lactic-co-glycolic acid) nanoparticles in vitro.
作者： Ronak Shabani , Mohsen Ashjari , Khadijeh Ashtari , Fariborz Izadyar , Babak Behnam
DOI: 10.2147/IJN.S155052
关键词: PLGA 、 Flow cytometry 、 Transplantation 、 Cytotoxicity 、 Cisplatin 、 Chemistry 、 In vitro 、 Apoptosis 、 Molecular biology 、 Cancer cell
摘要: Background Some male survivors of childhood cancer are suffering from azoospermia. In addition, spermatogonial stem cells (SSCs) necessary for the improvement spermatogenesis subsequent to exposure cytotoxic agents such as cisplatin. Objective The aim this study was evaluate anticancer activity cisplatin-loaded folic acid-conjugated poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) on mouse malignant cell line (EL4) and SSCs in vitro. Methods were co-cultured with divided into four culture groups: 1) control (cells medium), 2) treated cisplatin (10 μg/mL), 3) PLGA NPs, 4) co-cultures 48 hours. NPs prepared, characterized, targeted folate. vitro release characteristics, loading efficiency, scanning electron microscopy transmission images studied. Cancer assayed after treatment using flow cytometry TUNEL assay. EL4 injected seminiferous tubules testes treating cis-diaminedichloroplatinum/PLGA NPs. Results mean diameter ranged between 150 250 nm. number TUNEL-positive increased, expression Bax caspase-3 upregulated Group 4 compared 2. There no pathological tumor transplantation cells. Conclusion appeared act a promising carrier administration, which consistent higher activation apoptosis than free drug.
sci-hub.se 参考文章(23)
Hanna Valli, Kathrin Gassei, Kyle E. Orwig, Stem Cell Therapies for Male Infertility: Where Are We Now and Where Are We Going? Springer International Publishing. pp. 17- 39 ,(2015) , 10.1007/978-3-319-17849-3_3
Wim H De Jong, Paul JA Borm, Drug Delivery and Nanoparticles:applications and Hazards International Journal of Nanomedicine. ,vol. 3, pp. 133- 149 ,(2008) , 10.2147/IJN.S596
R. Shabani, K. Ashtari, B. Behnam, F. Izadyar, H. Asgari, M. Asghari Jafarabadi, M. Ashjari, E. Asadi, M. Koruji, In vitro toxicity assay of cisplatin on mouse acute lymphoblastic leukaemia and spermatogonial stem cells. Andrologia. ,vol. 48, pp. 584- 594 ,(2016) , 10.1111/AND.12490
Philip S Low, Asok C Antony, Folate receptor-targeted drugs for cancer and inflammatory diseases Advanced Drug Delivery Reviews. ,vol. 56, pp. 1055- 1058 ,(2004) , 10.1016/J.ADDR.2004.02.003
L. Marcon, X. Zhang, B. F. Hales, B. Robaire, M. C. Nagano, Effects of chemotherapeutic agents for testicular cancer on rat spermatogonial stem/progenitor cells. Journal of Andrology. ,vol. 32, pp. 432- 443 ,(2011) , 10.2164/JANDROL.110.011601
Mi Hou, Margareta Andersson, Chengyun Zheng, Anne Sundblad, Olle Söder, Kirsi Jahnukainen, Decontamination of leukemic cells and enrichment of germ cells from testicular samples from rats with Roser's T-cell leukemia by flow cytometric sorting. Reproduction. ,vol. 134, pp. 767- 779 ,(2007) , 10.1530/REP-07-0240
Sarbari Acharya, Sanjeeb K. Sahoo, PLGA nanoparticles containing various anticancer agents and tumour delivery by EPR effect Advanced Drug Delivery Reviews. ,vol. 63, pp. 170- 183 ,(2011) , 10.1016/J.ADDR.2010.10.008
Peisheng Xu, Edward A. Van Kirk, William J. Murdoch, Yihong Zhan, Dale D. Isaak, Maciej Radosz, Youqing Shen, Anticancer efficacies of cisplatin-releasing pH-responsive nanoparticles. Biomacromolecules. ,vol. 7, pp. 829- 835 ,(2006) , 10.1021/BM050902Y
Philip Stewart Low, Sumith Anurasiri Kularatne, Folate-targeted therapeutic and imaging agents for cancer. Current Opinion in Chemical Biology. ,vol. 13, pp. 256- 262 ,(2009) , 10.1016/J.CBPA.2009.03.022
Medha D Joshi, Vandana Patravale, Rashmi Prabhu, Polymeric nanoparticles for targeted treatment in oncology: current insights International Journal of Nanomedicine. ,vol. 10, pp. 1001- 1018 ,(2015) , 10.2147/IJN.S56932