Quantification of mRNA stability of stress-responsive yeast genes following conditional excision of open reading frames

摘要: Eukaryotic cells rapidly adjust the levels of mRNAs in response to environmental stress primarily by controlling transcription and mRNA turnover. How different conditions influence fate stress-responsive mRNAs, however, is relatively poorly understood. This largely due fact that half-life assays are traditionally based on interventions (e.g., temperature-shifts using temperature-sensitive RNA polymerase II alleles or treatment with general inhibitory drugs), which, rather than blocking, specifically induce genes. To study half-lives latter suite we developed describe here a minimally perturbing alternative method, coined CEO, which discontinuance following conditional excision open reading frames. Using confirm target rapamycin complex I (TORC1), nutrient-activated, central stimulator eukaryotic cell growth, favors decay depend stress- and/or nutrient-regulated factors Msn2/4 Gis1 for their transcription. We further demonstrate TORC1 controls stability these via Rim15-Igo1/2-PP2ACdc55 effector branch, reportedly also promoter recruitment. These data pinpoint PP2ACdc55 as node homo-directional coordination post-transcriptional stabilization specific array