DNA probes for mycobacteria. I. Isolation of DNA probes for the identification of Mycobacterium tuberculosis complex and for mycobacteria other than tuberculosis (MOTT).

摘要: Abstract Traditional methods used in identifying mycobacteria such as acid-fast bacillus stains and culture are often time-consuming, insensitive non-specific. The isolation of DNA probes, coupled to a non-radioactive, e.g. biotin-based detection system, have the potential foster development clinical assays for Mycobacterium tuberculosis other than (MOTT) that rapid, sensitive specific. To this end, we isolated two different probes: one which is specific Mtb complex recognizes all potentially pathogenic mycobacteria. use these probes combination should allow differentiation M. from MOTT. isolate first probe, prepared library fragments λ EMBL phage vector. Recombinant were screened by plaque-lift hybridization procedures using nick-translated mycobacterial genomic identify sequences complex. Inserts candidate recombinant purified, hybridized against wide variety filter-bound non-mycobacterial DNAs. Two clones identified closely related tuberculosis, bovis microti but not species second probe was preparing malmoense screening hybridization. One clone which, addition recognizing members complex, also intracellulare, malmoense, scrofulaceum, simiae, xenopi, avium, szulgai, kansasii haemophilum. None three species.