Method of construction of recombinant organisms using multiple genes co-integration

作者: Ju-Young Lee , Hyun-min Koo , Ji-Eun Kim , Jae-Chan Park , Soon-chun Chung

DOI:

关键词: Mixing (physics)Nucleic acidBiologyGrowth phaseRecombinant DNANucleic acid sequenceBiochemistryMicrobiologyOrganismGene

摘要: Provided is a method of simultaneous co-integration multiple nucleic acid sequences into microbial organism comprising culturing to be transformed until growth phase reached in which random integration facilitated; and transforming the by mixing cultured with sample co-integrated with, wherein comprises more than one sequence, products generated from method.

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