NUCLEOTIDE SEQUENCE OF 5'-UPSTREAM REGION AND EXPRESSION OF A SILKWORM GENE ENCODING A NEW MEMBER OF THE ATTACIN FAMILY

作者： Kiyoko Taniai , Takaaki Ishii , Masao Sugiyama , Akihiro Miyanoshita , Minoru Yamakawa

DOI: 10.1006/BBRC.1996.0448

关键词: Molecular biology 、 Biology 、 Nucleic acid sequence 、 TATA box 、 Gene 、 Genomic library 、 CAAT box 、 Genetics 、 Bombyx mori 、 Northern blot 、 Peptidoglycan

摘要: A genomic clone encoding a new member of attacin, an insect antibacterial protein, was isolated from library the silkworm, Bombyx mori, and nucleotide sequence 5′-upstream region determined. The contained Bm 1, highly repetitive element B. mori lipopolysaccharide (LPS) response (RE)(NF-κB binding site), CAAT box TATA box. Northern blot analysis showed that attacin gene expression rapidly induced by bacterial cell wall components such as LPS Escherichia coli peptidoglycan (PG) Micrococcus luteus, suggesting plays important role in early phase self-defense system upon infection.