Multi-metabolomics using imaging mass spectrometry and liquid chromatography-tandem mass spectrometry for spatially characterizing monoterpene indole alkaloids secreted from roots

摘要: Plants release specialized (secondary) metabolites from their roots to communicate with other organisms, including soil microorganisms. The spatial behavior of such around these can help us understand roles for the communication; however, currently they are unclear because soil-based studies complex. Here, we established a multi-metabolomics approach using imaging mass spectrometry (IMS) and liquid chromatography-tandem (LC-MS/MS) spatially assign under laboratory conditions agar. In case study Catharanthus roseus, showed that 58 nitrogen (N)-containing released into For metabolite assignment, used 15N-labeled nonlabeled LC-MS/MS data, previously reported. Four ions were identified authentic standard compounds as derived monoterpene indole alkaloids (MIAs) ajmalicine, catharanthine, serpentine, yohimbine. An alkaloid network analysis dot products spinglass methods characterized five clusters which belong. clustered indolic skeleton-type MIAs cluster, suggesting communities may represent distinct groups. future chemical assignments serpentine community, key fragmentation patterns MS/MS spectra.