Using omeprazole to link the components of the post-prandial alkaline tide in the spiny dogfish, Squalus acanthias.

摘要: After a meal, dogfish exhibit metabolic alkalosis in the bloodstream and marked excretion of basic equivalents across gills to external seawater. We used H(+), K(+)-ATPase pump inhibitor omeprazole determine whether these post-prandial alkaline tide events were linked secretion H(+) (accompanied by Cl(-)) stomach. Sharks fitted with indwelling stomach tubes for pretreatment (five doses 5 mg per kilogram over 48 h) or comparable volumes vehicle (saline containing 2% DMSO) sampling gastric chyme. Fish then fed an involuntary meal means tube consisting minced flatfish muscle (2% body mass) suspended saline (4% mass total volume). Omeprazole pre-treatment delayed acidification chyme, slowed rise Cl(-) concentration chyme altered patterns other ions, indicating inhibition accompanying secretion. also greatly attenuated arterial pH bicarbonate concentrations reduced net water 56% h. Arterial blood CO(2) pressure (Pa(CO(2))) plasma ions not substantially altered. These results indicate that elevated (as HCl) digestive process is major cause systemic base constitute dogfish.