Identification of Vibrio parahaemolyticus in Seafood by Multiplex PCR
作者： Chung-Saint Lin , Tser-Sheng Lin , Din-Yuan Yu , Yi-Cheng Su , Yung-Hsiang Tsai
DOI: 10.1080/10498850.2015.1056864
关键词: Thermostable direct hemolysin 、 Chitinase 、 Multiplex polymerase chain reaction 、 Vibrio parahaemolyticus 、 Human pathogen 、 Biology 、 Microbiology
摘要: ABSTRACTVibrio parahaemolyticus is a human pathogen frequently found in seafood. Once the seafood contaminated by V. parahaemolyticus, it can become vehicle for foodborne illness. The conventional culture methods detection of are time-consuming and cannot differentiate pathogenic strains from nonpathogenic ones. In this study, multiplex polymerase chain reaction (PCR) technique was investigated detecting tdh, chiA, toxR parahaemolyticus. sensitivity PCR determined testing 28 15 non-V. strains, fresh spiked with cells All were analyzed production thermostable direct hemolysin (TDH) chitinase. This study showed that both chiA excellent markers targeting tdh genes be applied to simultaneously detect environmental parahaem...
sci-hub.se 参考文章(26)
Leif Sundheim, Alan R. Poplawsky, Albert H. Ellingboe, Molecular cloning of two chitinase genes from Serratia marcescens and their expression in Pseudomonas species Physiological and Molecular Plant Pathology. ,vol. 33, pp. 483- 491 ,(1988) , 10.1016/0885-5765(88)90013-6
A K Bej, R J Steffan, J DiCesare, L Haff, R M Atlas, Detection of coliform bacteria in water by polymerase chain reaction and gene probes. Applied and Environmental Microbiology. ,vol. 56, pp. 307- 314 ,(1990) , 10.1128/AEM.56.2.307-314.1990
T Watanabe, W Oyanagi, K Suzuki, H Tanaka, Chitinase system of Bacillus circulans WL-12 and importance of chitinase A1 in chitin degradation. Journal of Bacteriology. ,vol. 172, pp. 4017- 4022 ,(1990) , 10.1128/JB.172.7.4017-4022.1990
Wei-Chih Cheng, Chia-Wei Kuo, Tsu-Yuan Chi, Lan-Chi Lin, Chen-Hsien Lee, Ruenn-Lan Feng, Shu-Jean Tsai, Investigation on the trend of food-borne disease outbreaks in Taiwan (1991–2010) Journal of Food and Drug Analysis. ,vol. 21, pp. 261- 267 ,(2013) , 10.1016/J.JFDA.2013.07.003
C Soumet, D Blivet, G Ermel, P Colin, G Salvat, An immunoconcentration-PCR assay to detect Salmonella in the environment of poultry houses. International Journal of Food Microbiology. ,vol. 48, pp. 221- 224 ,(1999) , 10.1016/S0168-1605(99)00049-5
T. Honda, T. Iida, The pathogenicity of Vibrio parahaemolyticus and the role of the thermostable direct haemolysin and related haemolysins Reviews in Medical Microbiology. ,vol. 4, pp. 106- 113 ,(1993) , 10.1097/00013542-199304000-00006
Hiroshi Tsujibo, Hideyuki Orikoshi, Chiaki Imada, Yoshiro Okami, Katsushiro Miyamoto, Yoshihiko Inamori, Site-directed mutagenesis of chitinase from Alteromonas sp. strain O-7. Bioscience, Biotechnology, and Biochemistry. ,vol. 57, pp. 1396- 1397 ,(1993) , 10.1271/BBB.57.1396
Shubha Gopal, Subhendu K. Otta, Sanath Kumar, Indrani Karunasagar, Matsuaki Nishibuchi, Iddya Karunasagar, The occurrence of Vibrio species in tropical shrimp culture environments; implications for food safety International Journal of Food Microbiology. ,vol. 102, pp. 151- 159 ,(2005) , 10.1016/J.IJFOODMICRO.2004.12.011
Asim K. Bej, Donald P. Patterson, Cynthia W. Brasher, Michael C.L. Vickery, Daniel D. Jones, Charles A. Kaysner, Detection of total and hemolysin-producing Vibrio parahaemolyticus in shellfish using multiplex PCR amplification of tl, tdh and trh Journal of Microbiological Methods. ,vol. 36, pp. 215- 225 ,(1999) , 10.1016/S0167-7012(99)00037-8
H.-C Wong, M.-C Chen, S.-H Liu, D.-P Liu, Incidence of highly genetically diversified Vibrio parahaemolyticus in seafood imported from Asian countries International Journal of Food Microbiology. ,vol. 52, pp. 181- 188 ,(1999) , 10.1016/S0168-1605(99)00143-9