Avaliação da acurácia e confiabilidade do teste sorológico de neutralização por redução de placas de lise (micro PRNT) na detecção de anticorpos para o vírus da Febre Amarela

摘要: Yellow fever is caused by the prototype virus of genus Flavivirus and it transmitted to humans through bite bloodsucking mosquitoes infected with viral agent. The disease remains endemic in tropical forest regions from Africa South America, despite availability effective vaccines against yellow - 17D-204 17DD considered safe highly immunogenic, capable inducing a rapid specific immune response, formation neutralizing antibodies that appear early, are protective long lasting. plaque reduction neutralization test (PRNT) most sensitive test for detection quantification antibodies, reference method assessing response after vaccination. This study evaluated reliability accuracy micro PRNT50 (applied 96-well plates) PRNT50 6-well compared performance the PRNT90. Two-hundred serum samples Brazilian individuals who participated in clinical dose-response vaccine produced Bio-Manguinhos were selected. repeatability was measured three independent repetitions each 200 sera dual operator same procedure performed other two operators, order determine reproducibility PRNT50. intraclass correlation coefficient (ICC) Pearson determined. Accuracy measures determined this sensitivity, specificity, overall accuracy, prevalence positive predictive values (PPV) negative (NPV). ROC curve also used best cut-off point PRNT50 . In assessment repeatability, ICCs operators ranged 0.62 0.76, coefficients between 0.78. For reproducibility, ICC obtained 0.72 coefficients 0.70 0.76. accuracy achieved considering 2.9 log10 mIU/mL, which had sensitivity 91.1%, specificity 72.9% 78%. The PRNT50 with ratified analysis. Considering hypothetical scenario an outbreak YF, prevalence would be 28%, PPV 56.7% NPV 95.5%. PRNT50 showed satisfactory levels of reliability however PRNT90 showed higher sensitivity 100%, 94.7% 95%. Modifications methodology changes classification criteria readings results obtained will important improve PRNT.