Production of galactooligosaccharides by β-galactosidase immobilized on glutaraldehyde-treated chitosan beads
作者： Dey-Chyi Sheu , Shin-Yi Li , Kow-Jen Duan , C. Will Chen
关键词: Lactose 、 Biochemistry 、 Chitosan 、 Aspergillus oryzae 、 Bioreactor 、 Glutaraldehyde 、 Oligosaccharide 、 Immobilized enzyme 、 Chemistry 、 Chromatography 、 Galactose
摘要: β-Galactosidase from Aspergillus oryzae, immobilized on glutaraldehyde-treated chitosan beads, produced galactooligosaccharides (GOS) in a plug reactor. Maximum yields of GOS were 18, 21, 26 with lactose at 100, 200, 300 g/L, respectively. Yields the enzyme system always lower than that free system. In continuous reaction there was no significant loss activity after 30 days operation.
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