Deciphering the role of stress responsive genes in chickpea (Cicer arietinum L.).

摘要: Chickpea, an important legume crop is seriously challenged by several abiotic and biotic stresses. Understanding the mechanisms that regulate expression of stress-related genes a fundamental issue necessary for genetic improvement chickpea. In separate studies based on in silico analysis Illumina GA Sanger sequencing data generated from drought Helicoverpa tissues contrasting parental genotypes provided differentially expressed stress-responsive are involved stress signaling pathways. This work presents deciphering role identified conferring resistance to infestation. A set 111 55 candidate insect tolerance, respectively, were targeted experimental validation using quantitative real time-PCR (qRT-PCR). Total RNA root (drought) leaf (Helicoverpa) stressed unstressed plants analysed context tolerant/sensitive genotypes. qRT-PCR confirmed predicted total 24 genes. includes 15 known 9 novel unreported terms cross-talking between under these two stresses three encoding mannitol dehydrogenase, NADPH quinine oxidoreductase cytochrome P-450 showed differential expression. Emerging evidence suggests pathways regulated ABA pathway, Oxidative phosphorylation pathway as well ROS play key roles crosstalk signaling. The could be used improve constraints affecting chickpea production.