Ultrasound-facilitated assembly and disassembly of a pH-sensitive self-assembly peptide

摘要: In this report, we investigated the impact of external stimulus (ultrasound) and internal (pH) on peptide assembly disassembly. Two short rationally designed peptides K3C6SPD F20H differing in presence a single pH-sensitive histidine residue were studied as model peptides. The kinetics studies demonstrated that substitution phenylalanine with (F20H) significantly slowed down rate at all three tested pHs (pH 9.5, pH 7.4 5.0). At same time, F to H led increased pH-responsive kinetics. By treating sample beginning process 9.5 for 5 min ultrasound power 2.1 W cm−2, was accelerated lag phase being shortened from 10 days 2 days. For disassembly nanofibrils preformed upon adjustment 5.0, ultrasonication resulted nanofibril within 6 hours, instead absence ultrasound. On contrary, similar treatment did not produce any obvious effect both processes. This study offers an effective strategy modulate stimuli-responsiveness peptide-based biomaterials.