QUANTIFICATION OF ASCORBIC ACID IN TROPICAL FRUITS BY A NEW METHOD USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND ION EXCHANGE COLUMN
作者： J. S. da Rosa , S. Pacheco , R. L. de O. Godoy , J. Oiano Neto
DOI:
关键词: Malpighia 、 Chromatography 、 Sulfuric acid 、 Oxalic acid 、 Ascorbic acid 、 Vitamin C 、 Chemistry 、 Extraction (chemistry) 、 Ion exchange 、 High-performance liquid chromatography
摘要: In this study, ascorbic acid (vitamin C) was quanti fied in some tropical fruits samples and potato using high performance liquid chromatography / ion exchange column sulfuric 0.05M as mobile phase. This method is faster than o thers used for vitamin C quantification which apply extraction with metaphosphoric or oxalic s reversed phase-liquid (RP- HPLC) buffered Interferences by colored substances phenolic compounds are absent when compared the traditionally ti trimetric method. KEYWORDS : exchange, acid, Solanum , Malpighia Spondias . INTRODUCTION Some exotic gaining a prominent role i n diet due to their nutritional value generally content of hydrosol uble vitamins, especially
cabdirect.org
researchgate.net 参考文章(9)
Allan R Brause, David C Woollard, Harvey E Indyk, J Acar, K Adadevoh, G Cherix, B Durst, T Eisele, E Elkins, J Foos, S Hammack, D Hammond, F Hesford, C Hischenhuber, V Hong, C J Huang, S Kirksey, L Kline, D Kruger, M J Lawson, A Lea, G Martin, A Parkih, J Weiss, E Wilhelmsen, B Woodward, R Wrolstad, L Zygmunt, , Determination of total vitamin C in fruit juices and related products by liquid chromatography: interlaboratory study. Journal of AOAC International. ,vol. 86, pp. 367- 374 ,(2003) , 10.1093/JAOAC/86.2.367
Samy H Ashoor, Woodrow C Monte, Jim Welty, Liquid chromatographic determination of ascorbic acid in foods. Journal - Association of Official Analytical Chemists. ,vol. 67, pp. 78- 80 ,(1984) , 10.1093/JAOAC/67.1.78
Glen L. Wheeler, Mark A. Jones, Nicholas Smirnoff, The biosynthetic pathway of vitamin C in higher plants Nature. ,vol. 393, pp. 365- 369 ,(1998) , 10.1038/30728
H. Greenfield, D. A. T. Southgate, Food Composition Data: Production, Management and Use ,(1992)
Giuliana Vinci, Francesco Botrè, Giustino Mele, Giovanni Ruggieri, Ascorbic acid in exotic fruits : a liquid chromatographic investigation Food Chemistry. ,vol. 53, pp. 211- 214 ,(1995) , 10.1016/0308-8146(95)90791-5
Gábor Bánhegyi, László Braun, Miklós Csala, Ferenc Puskás, József Mandl, Ascorbate Metabolism and Its Regulation in Animals Free Radical Biology and Medicine. ,vol. 23, pp. 793- 803 ,(1997) , 10.1016/S0891-5849(97)00062-2
William D. Graham, Donald Annette, Determination of ascorbic and dehydroascorbic acid in potatoes (Solanum tuberosum) and straberries using ion-exclusion chromatography Journal of Chromatography A. ,vol. 594, pp. 187- 194 ,(1992) , 10.1016/0021-9673(92)80329-S
Vural Gökmen, Nermin Kahraman, Nilay Demir, Jale Acar, Enzymatically validated liquid chromatographic method for the determination of ascorbic and dehydroascorbic acids in fruit and vegetables Journal of Chromatography A. ,vol. 881, pp. 309- 316 ,(2000) , 10.1016/S0021-9673(00)00080-7
A Rizzolo, A Polesello, Application of HPLC to the determination of water-soluble vitamins in foods: II (a review 1985-9) Journal of micronutrient analysis. ,vol. 8, pp. 105- 158 ,(1990)