Trypanosoma evansi: molecular homogeneity as inferred by phenetical analysis of ribosomal internal transcribed spacers DNA of an eclectic parasite.
作者： Aneska Norek de Oliveira Lima , Simone da Silva Santos , Heitor Miraglia Herrera , Carla Gama , Elisa Cupolillo
DOI: 10.1016/J.EXPPARA.2007.10.003
关键词: Restriction fragment length polymorphism 、 Riboprinting 、 Trypanosoma evansi 、 Restriction enzyme 、 Biology 、 Genetics 、 Ribosomal RNA 、 Polymerase chain reaction 、 Spacer DNA 、 Locus (genetics)
摘要: The protozoan Trypanosoma evansi is described as presenting high morphological and genetic similarities among the isolates despite its biological heterogeneity wide geographical distribution. PCR amplification of internal transcribed spacers ribosomal gene in combination with coding region 5.8S subunit further submitted to restriction enzymes digestion were carried out DNAs extracted from 41 T. strains isolated horses, dogs, coatis capybaras two distinct regions Brazilian Pantanal. We also used one isolate Africa, Asia b. brucei Africa. Analysis RFLP profiles yielded a unique "riboprinting" that does not vary intraspecifically. These results provide insights on organization showed ITS analysis by show similarity this locus parasite.
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