Labeling, detection and identification of newly synthesized proteomes with bioorthogonal non-canonical amino-acid tagging

作者: Daniela C Dieterich , Jennifer J Lee , A James Link , Johannes Graumann , David A Tirrell

DOI: 10.1038/NPROT.2007.52

关键词: Click chemistryBioorthogonal chemistryBioinformaticsProteomeTandem mass spectrometryIsotope-coded affinity tagProteomicsBiochemistryAvidinAmino acidChemistry

摘要: … most abundant amino acid (9.83% of all amino-acid residues… for methionine, an essential amino acid, and does not require … metabolic labeling with radioactive amino acids ( 35 S-…

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