In Silico Analysis of nsSNPs of Carp TLR22 Gene Affecting its Binding Ability with Poly I:C
作者： Vemulawada Chakrapani , Kiran D. Rasal , Sunil Kumar , Shibani D. Mohapatra , Jitendra K. Sundaray
DOI: 10.1007/S12539-017-0247-2
关键词: Ligand (biochemistry) 、 Genetics 、 MODELLER 、 Gene 、 Missense mutation 、 RNA 、 In silico 、 Mutant 、 Biology 、 Mutation 、 General Biochemistry, Genetics and Molecular Biology 、 Health informatics 、 Computer Science Applications
摘要: Immune response mediated by toll-like receptor 22 (TLR22), only found in teleost/amphibians, is triggered double-stranded RNA binding to its LRR (leucine-rich repeats) ecto-domain. Accumulated evidences suggested that missense mutations TLR genes affect function. However, information on mutation linked pathogen recognition for TLR22 was lacking. The present study commenced predicting the effect of non-synonymous single-nucleotide polymorphisms (nsSNPs) recognizable domain farmed carp, Labeo rohita. sequence-based algorithms (SIFT, PROVEAN and I-Mutant2.0) indicated three SNPs (out 27) such as p.L159F (rs76759876) p.L529P (rs749355507) LRR, p.I836M (rs750758397) intracellular motifs could potentially disrupt protein 3D structure generated using MODELLER 9.13 further validated SAVEs server. simulated molecular docking native mutants with poly I:C ligand positioned at region affects affinity significantly. This first kind nsSNPs teleost disturbed extra-cellular thereby likely hindrance subsequent signal transduction. serves a guide vivo evaluation impact immune gene.
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