Immunologic detection of retina cognin on the surface of embryonic cells

摘要: Abstract The retina cell-aggregating glycoprotein, referred to as the cognin, has been demonstrated be located at surface of embryonic neural cells. term cognin is used indicate its postulated role in mechanism mutual recognition and morphogenetic association Antiserum was prepared highly purified derived from isolated cell membranes chick embryo retina, it detect on cells embryos by means complement-mediated lysis. Retina (from 10-day embryos) freshly dissociated with trypsin showed little—if any—lysis antiserum; this consistent sensitivity trypsin. However, became susceptible immunolysis after a period incubation 37 °C, which indicates regeneration during recovery period. This required protein synthesis. Immunofluorescence tests binding antiserum recovered cells, thereby further demonstrating location cognin. presence, availability or ability regenerate assayed here, declined sharply age Addition exogenous trypsin-dissociated markedly increased their susceptibility antiserum, that added becomes associated these In contrast, addition trypsinized optic tectum cerebrum, 14-day did not increase antiserum. These results are earlier findings enhancement aggregation tissue-specific stage-specific. Cells non-neural tissues were lysed tissues, such tectum, found contain cross-reaction