Transcriptome-Wide Identification of miRNA Targets under Nitrogen Deficiency in Populus tomentosa Using Degradome Sequencing.

摘要: miRNAs are endogenous non-coding small RNAs with important regulatory roles in stress responses. Nitrogen (N) is an indispensable macronutrient required for plant growth and development. Previous studies have identified a variety of known novel responsive to low N plants, including Populus. However, involved the cleavage target genes corresponding networks response Populus remain largely unknown. Consequently, degradome sequencing was employed global detection validation N-responsive their targets. A total 60 unique (39 conserved, 13 non-conserved, eight novel) were experimentally 64 mRNA transcripts 21 precursors. Among them, we further verified 11 previously provided empirical evidence mode these on mRNAs. Furthermore, five miRNA stars (miRNA*s) shown function. The specificity diversity sites targets precursors P. tomentosa detected. Identification annotation miRNA-mediated can increase our understanding molecular mechanisms woody plants adapted environments.