Four Strains of Escherichia coli O157:H7 Isolated from Patients during an Outbreak of Disease Associated with Ground Beef: Importance of Evaluating Multiple Colonies from an Outbreak-Associated Product

作者: M. E. Proctor , T. Kurzynski , C. Koschmann , J. R. Archer , J. P. Davis

DOI: 10.1128/JCM.40.4.1530-1533.2002

关键词: DiseaseGenotypeFood poisoningOutbreakMicrobiologyPulsed field electrophoresisPulsed-field gel electrophoresisBiologyEscherichia coli

摘要: This report describes the investigation of a ground-beef-associated outbreak that involved five genetically distinct patient strains Escherichia coli O157:H7. Human and product isolates were evaluated by pulsed-field gel electrophoresis (PFGE) with two endonucleases. The multiple-strain etiology this underscores importance isolating evaluating multiple colonies from outbreak-related products comparing endonuclease PFGE patterns all human identified during periods. emphasizes interviewing confirmed suspected case patients period, regardless pattern their isolate, to confirm or rule out an epidemiologic link outbreak.

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