Effects of standardized extract of Ginkgo biloba leaves EGb761 on mitochondrial functions: mechanism(s) of action and dependence on the source of mitochondria and respiratory substrate.
作者： Giedre Baliutyte , Sonata Trumbeckaite , Rasa Baniene , Vilmante Borutaite , Adolfas Toleikis
DOI: 10.1007/S10863-014-9590-8
关键词: Inner membrane 、 Cell 、 Respiration 、 Apoptosis 、 Biology 、 Mitochondrion 、 Ginkgo biloba 、 Biochemistry 、 Oxidative phosphorylation 、 Cytochrome
摘要: In this work, the effects of standardized dry extract Ginkgo biloba leaves, EGb761, on respiration rat heart and liver mitochondria were investigated. We revealed uncoupling oxidative phosphorylation in by EGb761 which was not observed respiring pyruvate + malate; oxidation succinate inhibited concentration-dependently, almost completely at 1.00 mg/mL. Uncoupling effect found to be due increase H+ K+ permeability inner membrane is most likely mediated ATP/ADP-translocator proteins. depressed State 3 with malate (similarly mitochondria) (stronger than malate) but respiratory chain Complex IV; inhibition restored uncoupler indicating inhibitory action complexes preceding IV and/or substrate transport. Moreover, rapidly reduced pure cytochrome c. This property together reduction H2O2 accumulation may beneficial for cell prevention apoptosis protection cellular functions pathological situations.
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sci-hub.se 参考文章(45)
Bruce J. Diamond, Mary R. Bailey, Ginkgo biloba: indications, mechanisms, and safety. Psychiatric Clinics of North America. ,vol. 36, pp. 73- 83 ,(2013) , 10.1016/J.PSC.2012.12.006
Jurgita Barauskaite, Regina Grybauskiene, Ramune Morkuniene, Vilmante Borutaite, Guy C Brown, Tetramethylphenylenediamine protects the isolated heart against ischaemia-induced apoptosis and reperfusion-induced necrosis. British Journal of Pharmacology. ,vol. 162, pp. 1136- 1142 ,(2011) , 10.1111/J.1476-5381.2010.01110.X
Allan G. Gornall, Charles J. Bardawill, Maxima M. David, Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry. ,vol. 177, pp. 751- 766 ,(1949) , 10.1016/S0021-9258(18)57021-6
Elisabetta A. Tendi, Francesca Bosetti, S. Fenella DasGupta, Anna Maria Giuffrida Stella, Katy Drieu, Stanley I. Rapoport, Ginkgo biloba extracts EGb 761 and bilobalide increase NADH dehydrogenase mRNA level and mitochondrial respiratory control ratio in PC12 cells. Neurochemical Research. ,vol. 27, pp. 319- 323 ,(2002) , 10.1023/A:1014963313559
Yuan Luo, Ginkgo biloba neuroprotection: Therapeutic implications in Alzheimer's disease. Journal of Alzheimer's Disease. ,vol. 3, pp. 401- 407 ,(2001) , 10.3233/JAD-2001-3407
S. Mahadevan, Y. Park, Multifaceted therapeutic benefits of Ginkgo biloba L.: chemistry, efficacy, safety, and uses. Journal of Food Science. ,vol. 73, ,(2007) , 10.1111/J.1750-3841.2007.00597.X
Gerald P. Brierley, Kemal Baysal, Dennis W. Jung, Cation transport systems in mitochondria: Na+ and K+ uniports and exchangers Journal of Bioenergetics and Biomembranes. ,vol. 26, pp. 519- 526 ,(1994) , 10.1007/BF00762736
Vian Azzu, Nadeene Parker, Martin D. Brand, High membrane potential promotes alkenal-induced mitochondrial uncoupling and influences adenine nucleotide translocase conformation. Biochemical Journal. ,vol. 413, pp. 323- 332 ,(2008) , 10.1042/BJ20080321
Julius Liobikas, Dalia Marija Kopustinskiene, Adolfas Toleikis, What controls the outer mitochondrial membrane permeability for ADP: facts for and against the role of oncotic pressure. Biochimica et Biophysica Acta. ,vol. 1505, pp. 220- 225 ,(2001) , 10.1016/S0005-2728(01)00170-0
Virginie Rhein, Maria Giese, Ginette Baysang, Fides Meier, Stefania Rao, Kathrin L. Schulz, Matthias Hamburger, Anne Eckert, Ginkgo Biloba Extract Ameliorates Oxidative Phosphorylation Performance and Rescues Aβ-Induced Failure PLoS ONE. ,vol. 5, pp. e12359- ,(2010) , 10.1371/JOURNAL.PONE.0012359