Transcriptome profiling of Vitis amurensis , an extremely cold-tolerant Chinese wild Vitis species, reveals candidate genes and events that potentially connected to cold stress

摘要: Vitis amurensis Rupr. is an exceptional wild-growing (grape) species that can safely survive a wide range of cold conditions, but the underlying cold-adaptive mechanism associated with gene regulation poorly investigated. We have analyzed physiochemical and transcriptomic changes caused by stress in cold-tolerant accession, ‘Heilongjiang seedling’, Chinese wild V. amurensis. statistically determined total 6,850 cold-regulated transcripts were involved regulation, including 3,676 up-regulated 3,174 down-regulated transcripts. A global survey messenger RNA revealed skipped exon most prevalent form alternative spicing event. Importantly, we found splicing events increased prolonged stress. also identified thirty-eight major TF families some which previously unknown. Moreover, large number candidate pathways for metabolism or biosynthesis secondary metabolites to be regulated cold, potential importance coordinating tolerance growth development. Several heat shock proteins factors detected intensively cold-regulated. Furthermore, validated expression profiles 16 candidates using qRT-PCR further confirm accuracy RNA-seq data. Our results provide genome-wide view dynamic transcriptome amurensis, it evident various structural regulatory genes are crucial tolerance/adaptation. our robust dataset advances knowledge complex networks leads better understanding mechanisms this extremely species.