Long-term introgression of crop genes into wild sunflower populations

摘要: A strategy of DNA pooling aimed at identifying markers linked to quantitative trait loci (QTLs), ‘Sequential Bulked Typing’ (SBT), is presented. The method proposed consists in from consecutive pairs individuals ranked phenotypically, i.e., pools are formed with (1st, 2nd), (3rd, 4th),…, (N-1st, Nth). N/2 subsequently amplified using the polymerase chain reaction (PCR). If whole population typed number PCRs per marker halved respect individual typing (IT). But if this combined selective genotyping extreme savings can be further increased. Two cases considered: first one (SBT0), it assumed that only presence or absence a given allele ascertained pool; second (SBT1), differences between band intensities distinguished. theory estimate by maximum likelihood QTL effect and its position flanking behaviour IT SBT was studied stochastic computer simulation backcross F2 populations. Three percentages subpattern distinction (0, 50 100%) two sizes (n=1200 600) effects (a=0.1 0.25 standard deviations) were considered. SBT1 had same power as half costs all situations studied. Accuracy location not increased dense markers, opposed typing. As result useful for accurate but rather pick up genome regions containing QTLs least moderate effect. developed provides general theoretical framework deal any detecting QTLs.