Diversity of family GH46 chitosanases in Kitasatospora setae KM-6054
作者： Mina Zitouni , Pascal Viens , Mariana G. Ghinet , Ryszard Brzezinski
DOI: 10.1007/S00253-017-8517-9
关键词: Streptomycetaceae 、 Bacilli 、 Glycoside hydrolase 、 Sequence analysis 、 Gene 、 Sequence alignment 、 Chitosanase 、 Kitasatospora 、 Biochemistry 、 Biology
摘要: The genome of Kitasatospora setae KM-6054, a soil actinomycete, has three genes encoding chitosanases belonging to GH46 family. (csn1-3) were cloned in Streptomyces lividans and the corresponding enzymes purified from recombinant cultures. csn2 clone yielded two proteins (Csn2BH Csn2H) differing by presence carbohydrate-binding domain. Sequence analysis showed that Csn1 Csn2H canonical chitosanases, while Csn3 resembled bacilli. activity four was tested variety conditions on diverse chitosan forms, including highly N-deacetylated or complexed with humic polyphosphoric acid. Kinetic parameters also determined. These tests unveiled biochemical diversity among these peculiarity compared other enzymes. observed is discussed based structural 3D models sequence alignment. This first study all produced single microbial strain.
springer.com
paperity.org参考文章(50)
Marie-Ève Lacombe-Harvey, Tamo Fukamizo, Julie Gagnon, Mariana G. Ghinet, Nicole Dennhart, Thomas Letzel, Ryszard Brzezinski, Accessory active site residues of Streptomyces sp. N174 chitosanase FEBS Journal. ,vol. 276, pp. 857- 869 ,(2009) , 10.1111/J.1742-4658.2008.06830.X
A. Pelletier, J. Sygusch, Purification and Characterization of Three Chitosanase Activities from Bacillus megaterium P1. Applied and Environmental Microbiology. ,vol. 56, pp. 844- 848 ,(1990) , 10.1128/AEM.56.4.844-848.1990
N Pagé, D Kluepfel, F Shareck, R Morosoli, Effect of signal peptide alterations and replacement on export of xylanase A in Streptomyces lividans. Applied and Environmental Microbiology. ,vol. 62, pp. 109- 114 ,(1996) , 10.1128/AEM.62.1.109-114.1996
Qianqian Lyu, Yanhong Shi, Song Wang, Yan Yang, Baoqin Han, Wanshun Liu, David N.M. Jones, Weizhi Liu, Structural and biochemical insights into the degradation mechanism of chitosan by chitosanase OU01 Biochimica et Biophysica Acta (BBA) - General Subjects. ,vol. 1850, pp. 1953- 1961 ,(2015) , 10.1016/J.BBAGEN.2015.06.011
N R Gilkes, B Henrissat, D G Kilburn, R C Miller, R A Warren, Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families. Microbiological Research. ,vol. 55, pp. 303- 315 ,(1991) , 10.1128/MR.55.2.303-315.1991
T Watanabe, W Oyanagi, K Suzuki, H Tanaka, Chitinase system of Bacillus circulans WL-12 and importance of chitinase A1 in chitin degradation. Journal of Bacteriology. ,vol. 172, pp. 4017- 4022 ,(1990) , 10.1128/JB.172.7.4017-4022.1990
G P Schep, M G Shepherd, P A Sullivan, Purification and properties of a β-1,6-glucanase from Penicillium brefeldianum Biochemical Journal. ,vol. 223, pp. 707- 714 ,(1984) , 10.1042/BJ2230707
Pascal Viens, Marie-Ève Lacombe-Harvey, Ryszard Brzezinski, Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes Marine Drugs. ,vol. 13, pp. 6566- 6587 ,(2015) , 10.3390/MD13116566
Taichi E Takasuka, Christopher M Bianchetti, Yuki Tobimatsu, Lai F Bergeman, John Ralph, Brian G Fox, None, Structure‐guided analysis of catalytic specificity of the abundantly secreted chitosanase SACTE_5457 from Streptomyces sp. SirexAA‐E Proteins. ,vol. 82, pp. 1245- 1257 ,(2014) , 10.1002/PROT.24491
Luis A. Rivas, Vı́ctor Parro, Mercedes Moreno-Paz, Rafael P. Mellado, The Bacillus subtilis 168 csn gene encodes a chitosanase with similar properties to a Streptomyces enzyme Microbiology. ,vol. 146, pp. 2929- 2936 ,(2000) , 10.1099/00221287-146-11-2929