Theophylline IMAGEtags (Intracellular Multi Aptamer Genetic tags): the development and evaluation of an RNA reporter system based on the theophylline aptamer
作者： Hans Eirik Haarberg , None
关键词: Biochemistry 、 Oligonucleotide 、 Tandem repeat 、 Protein domain 、 Cloning 、 Riboswitch 、 Aptamer 、 Biology 、 Reporter gene 、 RNA
摘要: iv CHAPTER 1. GENERAL INTRODUCTION 1 Current reporter genes Fluorescent proteins 3 In Vivo Imaging 6 RNA 8 Aptamers 9 Aptamer structure and stabilization 10 Applications of aptamers 11 Riboswitches natural RNAs with aptamer-like properties 12 Application for imaging 13 Sulforhodamine-B fluorescein Fluorescence switches “Light-up” 14 Theophylline aptamer 17 The theophylline 19 IMAGEtag system 22 Collaborator contributions 2. CLONING OF MULTIPLE TANDEM REPEATS PROTEIN DOMAINS AND APTAMERS 24 Abstract Introduction 25 Materials Methods 27 Cloning initial repetitive sequences from synthetic oligonucleotides Construction longer 28 Results Discussion Repetitive constructed using Multiplication the original 29 Other constructs 31 Stability Summary 32
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