Comparison of techniques to examine the diversity of fungi in adult patients with cystic fibrosis.
作者： Yuriko Nagano , J. Stuart Elborn , B. Cherie Millar , James M. Walker , Colin E. Goldsmith
DOI: 10.3109/13693780903127506
关键词: Aspergillus fumigatus 、 Transplantation 、 Candida albicans 、 Microbiological culture 、 Penicillium 、 Cladosporium 、 Microbiology 、 Biology 、 Exophiala 、 Aspergillus versicolor
摘要: This study compares conventional and molecular techniques for the detection of fungi in 77 adult cystic fibrosis (CF) patients. Three different methods were investigated, i.e., (1) microbiological culture (including yeasts filamentous fungi), (2) mycological with CF-derived fungal specific media, (3) Non-culture direct DNA extraction from patient sputa. Fungi isolated environmental air samples CF unit compared to sputa (n = 107) detected 14/77(18%) patients by method 1, 60/77 (78%) 2 3, 77/77(100%) The majority Candida albicans C. dubliniensis. Exophiala (Wangiella) dermatitidis, Scedosporium apiospermum, Penicillium spp., Aspergillus fumigatus, versicolor also identified sequence analysis rDNA short internal transcribed spacer (ITS2) region. Conventional laboratory failed de...
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