Quantitative Determination of Alkaloids in Lotus Flower (Flower Buds of Nelumbo nucifera) and Their Melanogenesis Inhibitory Activity
作者： Toshio Morikawa , Niichiro Kitagawa , Genzoh Tanabe , Kiyofumi Ninomiya , Shuhei Okugawa
DOI: 10.3390/MOLECULES21070930
关键词: Alkaloid 、 Benzylisoquinoline 、 Petal 、 Acetic acid 、 Chemistry 、 Chromatography 、 Coclaurine 、 Nuciferine 、 Carbamic acid 、 Stereochemistry 、 Electrospray ionization
摘要: A quantitative analytical method for five aporphine alkaloids, nuciferine (1), nornuciferine (2), N-methylasimilobine (3), asimilobine (4), and pronuciferine (5), benzylisoquinoline armepavine (6), norarmepavine (7), N-methylcoclaurine (8), coclaurine (9), norjuziphine (10), identified as the constituents responsible melanogenesis inhibitory activity of extracts lotus flowers (the flower buds Nelumbo nucifera), has been developed using liquid chromatography-mass spectrometry. The optimum conditions separation detection these 10 alkaloids were achieved on a πNAP column, reversed-phase column with naphthylethyl group-bonded silica packing material, CH3CN–0.2% aqueous acetic acid mobile phase mass spectrometry equipped positive-mode electrospray ionization source. According to protocol established, distributions in petal, receptacle, stamen parts, which separated from whole flower, examined. As expected, excellent correlations observed between total alkaloid content activity. Among active (2) was found give carbamate salt (2′′) via formation an unstable carbamic (2′) by absorption carbon dioxide air.
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