Antibodies against a Plasmodium falciparum RON12 inhibit merozoite invasion into erythrocytes.
作者： Daisuke Ito , Eizo Takashima , Tsutomu Yamasaki , Shinya Hatano , Tomoyuki Hasegawa
DOI: 10.1016/J.PARINT.2018.10.006
关键词: Biology 、 Rhoptry neck 、 Immunoelectron microscopy 、 Plasmodium falciparum 、 Molecular biology 、 Antibody 、 Immunofluorescence 、 Recombinant DNA 、 In vitro 、 Antigen
摘要: Abstract Proteins coating Plasmodium merozoite surface and secreted from its apical organelles are considered as promising vaccine candidates for blood-stage malaria. The rhoptry neck protein 12 of falciparum (PfRON12) was recently reported a specifically expressed in schizonts localized to the merozoites. Here, we assessed potential candidate. We recombinant PfRON12 by wheat germ cell-free system obtain anti-PfRON12 antibody. Immunoblot analysis schizont lysates detected single band at approximately 40 kDa under reducing conditions, consistent with predicted molecular weight. Additionally, antibody recognized around 80 kDa non-reducing suggesting native forms disulfide-bond-mediated multimer. Immunofluorescence assay immunoelectron microscopy revealed that merozoites free biological activity tested vitro growth inhibition (GIA), rabbit antibodies significantly inhibited invasion erythrocytes. then investigated whether is immunogenic P. falciparum-infected individuals. sera infected individuals Thailand Mali reacted PfRON12. Furthermore, human affinity-purified Malian serum samples erythrocytes vitro. Moreover, pfron12 highly conserved only 4 non-synonymous mutations coding sequence 200 isolates deposited PlasmoDB. These results suggest might be candidate antigen against falciparum.
sci-hub.se 参考文章(18)
Ellen Knuepfer, Oniz Suleyman, Anton R. Dluzewski, Ursula Straschil, Aisling H. O'Keeffe, Solabomi A. Ogun, Judith L. Green, Munira Grainger, Rita Tewari, Anthony A. Holder, RON12, a novel Plasmodium‐specific rhoptry neck protein important for parasite proliferation Cellular Microbiology. ,vol. 16, pp. 657- 672 ,(2014) , 10.1111/CMI.12181
Wandy L. Beatty, Ababacar Diouf, Raul Herrera, Xavier Ambroggio, J. Kathleen Moch, Jessica S. Tyler, David L. Narum, Susan K. Pierce, John C. Boothroyd, J. David Haynes, Louis H. Miller, Prakash Srinivasan, Binding of Plasmodium merozoite proteins RON2 and AMA1 triggers commitment to invasion Proceedings of the National Academy of Sciences of the United States of America. ,vol. 108, pp. 13275- 13280 ,(2011) , 10.1073/PNAS.1110303108
W Trager, J. Jensen, Human malaria parasites in continuous culture Science. ,vol. 193, pp. 673- 675 ,(1976) , 10.1126/SCIENCE.781840
Denise L. Doolan, Yunxiang Mu, Berkay Unal, Suman Sundaresh, Siddiqua Hirst, Conrad Valdez, Arlo Randall, Douglas Molina, Xiaowu Liang, Daniel A. Freilich, J. Aggrey Oloo, Peter L. Blair, Joao C. Aguiar, Pierre Baldi, D. Huw Davies, Philip L. Felgner, Profiling Humoral Immune Responses to P. falciparum Infection with Protein Microarrays Proteomics. ,vol. 8, pp. 4680- 4694 ,(2008) , 10.1002/PMIC.200800194
Jun Cao, Osamu Kaneko, Amporn Thongkukiatkul, Mayumi Tachibana, Hitoshi Otsuki, Qi Gao, Takafumi Tsuboi, Motomi Torii, None, Rhoptry neck protein RON2 forms a complex with microneme protein AMA1 in Plasmodium falciparum merozoites Parasitology International. ,vol. 58, pp. 29- 35 ,(2009) , 10.1016/J.PARINT.2008.09.005
Alan F. Cowman, Brendan S. Crabb, Invasion of Red Blood Cells by Malaria Parasites Cell. ,vol. 124, pp. 755- 766 ,(2006) , 10.1016/J.CELL.2006.02.006
Daisuke Ito, Tomoyuki Hasegawa, Kazutoyo Miura, Tsutomu Yamasaki, Thangavelu U. Arumugam, Amporn Thongkukiatkul, Satoru Takeo, Eizo Takashima, Jetsumon Sattabongkot, Eun-Taek Han, Carole A. Long, Motomi Torii, Takafumi Tsuboi, RALP1 Is a Rhoptry Neck Erythrocyte-Binding Protein of Plasmodium falciparum Merozoites and a Potential Blood-Stage Vaccine Candidate Antigen Infection and Immunity. ,vol. 81, pp. 4290- 4298 ,(2013) , 10.1128/IAI.00690-13
Elissa M. Malkin, David J. Diemert, Julie H. McArthur, John R. Perreault, Aaron P. Miles, Birgitte K. Giersing, Gregory E. Mullen, Andrew Orcutt, Olga Muratova, May Awkal, Hong Zhou, Jin Wang, Anthony Stowers, Carole A. Long, Siddhartha Mahanty, Louis H. Miller, Allan Saul, Anna P. Durbin, Phase 1 Clinical Trial of Apical Membrane Antigen 1: an Asexual Blood-Stage Vaccine for Plasmodium falciparum Malaria Infection and Immunity. ,vol. 73, pp. 3677- 3685 ,(2005) , 10.1128/IAI.73.6.3677-3685.2005
Tatiana M Lopera-Mesa, Saibou Doumbia, Drissa Konaté, Jennifer M Anderson, Mory Doumbouya, Abdoul S Keita, Seidina A S Diakité, Karim Traoré, Michael A Krause, Ababacar Diouf, Samuel E Moretz, Gregory S Tullo, Kazutoyo Miura, Wenjuan Gu, Michael P Fay, Steve M Taylor, Carole A Long, Mahamadou Diakité, Rick M Fairhurst, Effect of red blood cell variants on childhood malaria in Mali: a prospective cohort study The Lancet Haematology. ,vol. 2, pp. 140- ,(2015) , 10.1016/S2352-3026(15)00043-5
Takafumi Tsuboi, Satoru Takeo, Hideyuki Iriko, Ling Jin, Masateru Tsuchimochi, Shusaku Matsuda, Eun-Taek Han, Hitoshi Otsuki, Osamu Kaneko, Jetsumon Sattabongkot, Rachanee Udomsangpetch, Tatsuya Sawasaki, Motomi Torii, Yaeta Endo, Wheat germ cell-free system-based production of malaria proteins for discovery of novel vaccine candidates. Infection and Immunity. ,vol. 76, pp. 1702- 1708 ,(2008) , 10.1128/IAI.01539-07