Gene network and canonical pathway analysis in canine myxomatous mitral valve disease: a microarray study.

摘要: Abstract Myxomatous mitral valve disease (MMVD) is the single most common acquired heart of dog and particularly in small pedigree breed dogs such as Cavalier King Charles spaniel (CKCS). There are limited data on transcriptome aim this study was to use microarray technology conjunction with bioinformatics platforms analyse transcript changes MMVD CKCS compared normal (non-CKCS). Differentially expressed genes ( n  = 5397) were identified using cut-off settings fold change, false discovery rate (FDR) P  0.05. In total, 4002 annotated a specific Affymetrix canine database, after further filtering, 591 identified: 322 (55%) up-regulated 269 (45%) down-regulated. Canine microRNAs (cfa-miR;  = 59) also identified. Gene ontology network analysis between six 10 significantly different biological function clusters from which following selected relevant MMVD: inflammation, cell movement, cardiovascular development, extracellular matrix organisation epithelial-to-mesenchymal (EMT) transition. Ingenuity Pathway Analysis three canonical pathways caveolar-mediated endocytosis, remodelling epithelial adherens junctions, endothelin-1 signalling. Considering relevance MMVD, gene families importance significant difference groups included collagens, ADAMTS peptidases, proteoglycans, metalloproteinases (MMPs) their inhibitors, basement membrane components, cathepsin S, integrins, tight junction adhesion proteins, cadherins, other matrix-associated members serotonin (5-HT)/transforming growth factor -β signalling pathway.