Neurite outgrowth can be modulated in vitro using a tetracycline-repressible gene therapy vector expressing human nerve growth factor.

摘要: The delivery of neurotrophic factors to the adult nervous system has potential applications for treatment neurodegenerative diseases and trauma. In vivo ex gene therapy offer a means delivering growth other therapeutic substances central (CNS) in an intraparenchymal, accurately targeted, regionally restricted manner. Ideally, systems should also be regulatable, allowing exogenous control amount product delivery. present experiment, tetracycline-regulatable expression was generated determine whether controllable release nerve factor (NGF) green fluorescent protein (GFP) from primary rat fibroblasts could modulate biological responses (neurite outgrowth) vitro. Using tetracycline-repressible construct, it found that NGF mRNA, protein, NGF-induced neurite outgrowth tightly regulated within 24 hour period, dose-dependent fashion, by exposure tetracycline analog doxycycline. Similarly, levels fluorescence GFP-transfected cells. These findings neurobiological lay framework future studies using neurotrophin models CNS injury.