DIFFERENT ROLES OF TUMOUR NECROSIS FACTOR α AND INTERLEUKIN 1 IN MURINE STREPTOCOCCAL CELL WALL ARTHRITIS

摘要: In this study two different aspects of tumour necrosis factor alpha (TNF-alpha) and interleukin 1 (IL-1) in locally induced murine streptococcal cell wall arthritis (SCW) were investigated. First, the kinetics interdependence TNF-alpha IL-1 release; second; their involvement inflammation cartilage destruction. Kinetic studies showed that peak level preceded level. However, vivo neutralization did not result decreased bioactivity or immunoreactivity, suggesting there is no dominant TNF-alpha-dependent release model. Inflammation was studied by measuring knee joint swelling inflammatory influx. Impact on chondrocyte proteoglycan synthesis depletion. The role these phenomena investigated using anti-TNF-alpha antibodies binding protein (TNFbp). Similarly, anti-IL-1 receptor antagonist (IL-1Ra). Anti-TNF-alpha treatment significantly reduced swelling, whereas effect found IL-1Ra. contrast, IL-1, but TNF-alpha, resulted a significant decrease inhibition. Moreover, histology revealed depletion Combined anti-TNF-alpha/anti-IL-1 suppressed both damage. impact separate parameters exceed effects either anti-TNF-1. It can be concluded exert specific activities SCW arthritis. model limited to plays destruction