Constitutive hyperproduction of sorbicillinoids in Trichoderma reesei ZC121.

摘要: In addition to its outstanding cellulase production ability, Trichoderma reesei produces a wide variety of valuable secondary metabolites, the which has not received much attention date. Among them, sorbicillinoids, large group hexaketide metabolites derived from polyketides, are drawing growing interest researchers because they exhibit important biological functions, including anticancer, antioxidant, antiviral, and antimicrobial properties. The development fungi strains with constitutive, hyperproduction sorbicillinoids is thus desired for future industry application but well-studied. Moreover, although T. been demonstrated produce corresponding gene cluster biosynthesis pathway proposed, underlying molecular mechanism governing sorbicillinoid remains unknown. Recombinant ZC121 was constructed strain RUT-C30 by insertion 12121-knockout cassette at telomere chromosome IV in consideration off-target mutagenesis encountered during unsuccessful deletion 121121. Strain ZC121, when grown on cellulose, showed sharp reduction production, yet remarkable enhancement as compared RUT-C30. constitutive process, independent culture conditions such carbon source, light, pH, temperature. To best our knowledge, displays record levels both glucose cellulose. Sorbicillinol bisvertinolone two major compounds produced. displayed different morphology markedly reduced sporulation had similar growth rate biomass. Transcriptome analysis that most genes involved were downregulated significantly whereas remarkably all upregulated cellulose glucose. A sorbicillinoid-hyperproduction obtained mutagenesis, displaying an overwhelming shift leading production. For first time, degraded platform chemical other than protein high yield. We propose occurring region might cause remodeling subsequently alter cell structure global expression pattern shown phenotype profiling comparative transcriptome ZC121. Overall, holds great promise industrial serves good model explore regulation sorbicillinoids’ biosynthesis.