Determination of the cephalosporin Ro 13‐99041 in plasma, urine, and bile by means of ion‐pair reversed phase chromatography

摘要: An HPLC method has been developed for the determination of cephalosporin antibiotic Ro 13-9904 in plasma, urine, and bile dogs human volunteers using technique ion-pair chromatography with a LiChrosorb RP-18 column. The three mobile phases employed contained tetrapentyl-, tetraoctyl- hexadecyltrimethyl-ammonium bromide, respectively, as lipophilic counterions. chromatographic conditions chosen allowed simple rapid sample preparation. Plasma was deproteinized ethanol supernatant directly injected onto column; urine were diluted phase without any purification. detection limit about 0.5 μg/ml plasma samples approximately 5 urine.