Establishment of an In Vivo Model for Pediatric Ewing Tumors by Transplantation into NOD/ scid Mice

摘要: Ewing tumors are a clinically heterogeneous group of childhood sarcomas that represent paradigm for understanding solid tumor biology, as they the first which chromosome translocation has been characterized at molecular level. However, biologic organization tumor, especially processes govern proliferation, differentiation, and metastasis primitive stem cells is poorly understood. Therefore, to develop biologically relevant in vivo model, five different cell lines primary from three patients were transplanted into immune-deficient mice via intravenous injection. NOD/scid carry complex immune deficiency thus nearly completely lack ability reject human used recipients. Overall, 26 52 (50%) with VH-64, WE-68, CADO-ES1, TC-71, RM-82 4 10 (40%) engrafted. Moreover, did not grow vitro proliferated mice. The pattern was similar frequent metastases lungs (62%), bone marrow (30%), (23%). Using limiting dilution experiments, frequency engraftment unit estimated 1 tumor-initiating 3 x 10(5) VH-64 cells. These data demonstrate we have able establish an model recapitulates many aspects growth progression tumors. For time, this provides opportunity identify characterize clonogenic This will, therefore, be instrumental studying including organ-selective angiogenesis.