An efficient protocol for regenerating shoots from paper mulberry (Broussonetia papyrifera) leaf explants
作者： Siming Cui , Ying Ren , Yahan Hao , Junjie Zhang , Zhouchao Chen
关键词: Broussonetia 、 Explant culture 、 Biology 、 Tree species 、 Plant growth 、 Paper mulberry 、 Horticulture 、 Murashige and Skoog medium 、 Shoot 、 Plant science
摘要: Paper mulberry (Broussonetia papyrifera) is a tree species that has many economic, ecological, and social uses. This study developed an efficient protocol for regenerating shoots from leaf explants using Murashige Skoog (MS) medium supplemented with different concentrations of plant growth regulators (PGRs), which play vital roles in shoot regeneration. The best result, 86.67% induction frequency 4.35 per explant, was obtained the MS containing 2.0 mg/L N6-benzyladenine (BA) 0.05 indole-3-butyric acid. effects explant age, orientation, genotype were also investigated. Explants young leaves had greater regeneration than those old leaves, results better when distal end contacted versus proximal end. Approximately 70.96% rooted well 0.4 α-naphthalene acetic acid (NAA). Although some genotypes achieved poorer results, still applicable mass multiplication genetic transformation.
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