A Modified Method for Implantation of Pluripotent Stem Cells Under the Rodent Kidney Capsule
作者： Fuli Zhu , Bin Sun , Yan Wen , Zhe Wang , Renee Reijo Pera
关键词: Immunology 、 Reporter gene 、 Bioluminescence imaging 、 In vivo 、 Transplantation 、 Stem cell 、 Cell 、 Cell biology 、 Biology 、 Induced pluripotent stem cell 、 Embryonic stem cell
摘要: Teratoma formation, the standard in vivo pluripotency assay, is also frequently used as a tumorigenicity assay. A common concern therapeutic stem cell applications potential of small number impurities final product. Estimation this hampered by inaccurate methodology Hence, protocol for assay that can deliver defined cells, without error introduced leakage or migration cells needed. In study, we tested our modified transplantation method allows transplant numbers pluripotent (PSCs) under kidney capsule with minimal leakage. glass capillary finely shaped tip and an attached mouth pipette was to inject PSCs into rodent capsule. H9 embryonic induced were tagged Fluc green fluorescence protein reporter genes divided different doses transplantation. Bioluminescence imaging (BLI)...
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