Sensitive detection of chromosome copy number aberrations in prostate cancer by fluorescence in situ hybridization.

摘要: The pattern of chromosomal aberrations and their significance in prostate cancer are poorly understood. We studied 23 10 benign prostatic hyperplasia (BPH) specimens by fluorescence situ hybridization (FISH) using pericentromeric repeat-specific probes for different chromosomes. aims the study were: 1) to compare sensitivity FISH DNA flow cytometry aneuploidy detection, 2) determine which chromosome copy number changes most common, 3) probe combinations would be effective diagnosis. Disaggregated tumor cells from formalin-fixed, paraffin-embedded tissues were pretreated with our newly developed method based on hot glycerol solution improve penetration. All BPH diploid showed no numerical FISH. In cancer, abnormal content 35% cases, whereas 74% Aberrant chromosomes 8 (48% cases), X (43% 7 (39% cases) common. Ninety-four percent all aneuploid cases have been detected these three alone. Simple losses uncommon but tetraploid tumors relative (trisomy or disomy) several often found, suggesting progression through tetraploidization followed selected conclusion, results indicate that chromosome-specific is two times more sensitive than cytometric analysis detection.