Development of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) fibers for skin tissue engineering: effects of topography, mechanical, and chemical stimuli.

摘要: Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), a biodegradable polyester, was electrospun to form defect-free fibers with high surface-area-to-volume ratio for skin regeneration. Several parameters such as solvent ratio, polymer concentration, applied voltage, flow rate, and tip-to-target distance were optimized achieve morphology. The average diameter of the PHBV 724 ± 91 nm. also solvent-cast 2-D films, its mechanical properties, porosity, degradation rates compared fibers. Our results demonstrate that exhibited higher increased ductility, faster rate when films (p 0.05). gene expression collagen I elastin significantly up-regulated TCPS control, whereas III down-regulated on film after 14 days in culture. less ductile showed levels expression. Furthermore, presence absence an angiogenesis factor (R-Spondin 1) evaluated their wound healing capacity rat model. contracture R-Spondin-1-loaded found be alone 7 < promoted increase aided re-epithelialization. Thus our topography chemical stimuli have pronounced influence cell proliferation, expression, healing.