Two tropinone reducing enzymes from Datura stramonium transformed root cultures

摘要: Abstract Two different tropinone reductases (TR) were isolated from transformed root cultures of Datura stramonium. Both activities completely separated by hydrophobic interaction chromatography and affinity chromatography. Gas chromatographic analysis the reaction products showed one enzyme activity forming tropine only (TR I) other exclusively pseudotropine II). TR I after extraction purification about five-fold II. Characterization both enzymes revealed differences to previously stramonium, D. innoxia Hyoscyamus niger, between individual proteins. a pronounced pH-dependency while II was more tolerant pH-values. accepted NADPH as coenzyme; Km values 1.39 mM 0.22 II) for 59 μM 17 NADPH.