Quantitation of the Transfer of Surface Phospholipid of Chylomicrons to the High Density Lipoprotein Fraction during the Catabolism of Chylomicrons in the Rat

摘要: Small chylomicrons (CM) labeled with cholesterol, cholesterol ester, phospholipid, and, in some cases, protein, were used to study the fate of these constituents as CM are catabolized circulations hepatectomized and intact rat. In animal after (1/2) h, greatly reduced volume, surface area, diameter. During this period, lost >92% mass their triacylglycerol, >77% >39% protein. Compared injected CM, chemically altered particles, called "remnants," have a reduction volume 96% area 88%. The esters remain remnants but, strikingly, major fraction phospholipids soluble apoproteins leave found high density lipoprotein (HDL) fraction. chemical composition HDL contains relatively more phospholipid less ester than normal rat HDL. Because difference between rats those given we estimate that pool increased by congruent with25% infusion 4-5 mg phospholipid. Approximately 5 is secreted on fed 1 h. findings indicate minor protein (soluble non-B apoproteins) newly transferred from during remnant formation. same process probably occurs except particles rapidly removed plasma liver smaller enters